A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior.

@article{Clarkson1999AHF,
  title={A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior.},
  author={Michael Clarkson and Robert E. Saint},
  journal={DNA and cell biology},
  year={1999},
  volume={18 6},
  pages={
          457-62
        }
}
We have generated Drosophila melanogaster lines carrying a modified genomic fragment which encodes the D. melanogaster variant H2A.F/Z class histone, His2AvD, fused to the green fluorescent protein (GFP) of the jellyfish Aequorea victoria. We show here that the fusion protein consists of functional GFP and functional histone His2AvD. The His2AvD portion of the fusion gene was shown to be functional by rescue of His2AvD mutant lethality. Fluorescence of the fusion protein in vivo was observed in… CONTINUE READING
Highly Influential
This paper has highly influenced 13 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
Highly Cited
This paper has 140 citations. REVIEW CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 87 extracted citations

Chk1 and Wee1 kinases coordinate DNA replication, chromosome condensation, and anaphase entry

Molecular biology of the cell • 2012
View 4 Excerpts
Method Support
Highly Influenced

Greatwall kinase

The Journal of cell biology • 2004
View 4 Excerpts
Method Support
Highly Influenced

The dynamics of acentric chromosomes in cancer cells revealed by GFP-based chromosome labeling strategies.

Journal of cellular biochemistry. Supplement • 2000
View 5 Excerpts
Method Support
Highly Influenced

141 Citations

051015'00'04'09'14'19
Citations per Year
Semantic Scholar estimates that this publication has 141 citations based on the available data.

See our FAQ for additional information.