A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I

@inproceedings{Xu2011AHS,
  title={A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I},
  author={Hui Xu and Shuli Gao and Quanwen Liu and Dun Pan and Lihua Wang and Shuzhen Ren and Min Ding and Jingwen Chen and Gang Liu},
  booktitle={Sensors},
  year={2011}
}
We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+ -thymine (T-Hg2+ -T) complex. Cys can selectively coordinate with Hg2+ and extract it from the thymine-Hg2+ -thymine complex. The hairpin structure dehybridizes and the fluorescence intensity of Sybr Green I (SG) decreases… CONTINUE READING
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