A High-throughput Approach for Subcellular Proteome

  title={A High-throughput Approach for Subcellular Proteome},
  author={Xiao-Sheng Jiang and Hu Zhou and Lei Zhang and Qingxuan Sheng and Su-Jun Li and Long Li and Pei Hao and Yixue Li and Q Y Xia and Jia-Rui Wu and Rong Zeng},
Four fractions from rat liver (a crude mitochondria (CM) and cytosol (C) fraction obtained with differential centrifugation, a purified mitochondrial (PM) fraction obtained with nycodenz density gradient centrifugation, and a total liver (TL) fraction) were analyzed with two-dimensional liquid chromatography tandem mass spectrometry analysis. A total of 564 rat proteins were identified and were bioinformatically annotated according to their physicochemical characteristics and functions. While… CONTINUE READING


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