A High-Throughput Strategy for Dissecting Mammalian Genetic Interactions

@inproceedings{Stockman2016AHS,
  title={A High-Throughput Strategy for Dissecting Mammalian Genetic Interactions},
  author={Victoria B Stockman and Lila Ghamsari and Gorka Lasso and Barry Honig and Sagi D. Shapira and Harris H. Wang},
  booktitle={PloS one},
  year={2016}
}
Comprehensive delineation of complex cellular networks requires high-throughput interrogation of genetic interactions. To address this challenge, we describe the development of a multiplex combinatorial strategy to assess pairwise genetic interactions using CRISPR-Cas9 genome editing and next-generation sequencing. We characterize the performance of… CONTINUE READING