A High Throughput Screen for RGS Proteins Using Steady State Monitoring of Free Phosphate Formation

@inproceedings{Monroy2013AHT,
  title={A High Throughput Screen for RGS Proteins Using Steady State Monitoring of Free Phosphate Formation},
  author={Carolina Monroy and Duncan I Mackie and David L{\'a}zaro Rom{\'a}n},
  booktitle={PloS one},
  year={2013}
}
G-protein coupled receptors are a diverse group that are the target of over 50% of marketed drugs. Activation of these receptors results in the exchange of bound GDP for GTP in the Gα subunit of the heterotrimeric G-protein. The Gα subunit dissociates from the β/γ subunits and both proceed to affect downstream signaling targets. The signal terminates by the hydrolysis of GTP to GDP and is temporally regulated by Regulators of G-protein Signaling (RGS) proteins that act as GTPase Activating… CONTINUE READING
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