A High-Throughput Method for Screening of Rapamycin-Producing Strains of Streptomyces hygroscopicus by Cultivation in 96-Well Microtiter Plates

@article{Xu2005AHM,
  title={A High-Throughput Method for Screening of Rapamycin-Producing Strains of Streptomyces hygroscopicus by Cultivation in 96-Well Microtiter Plates},
  author={Zhi-nan Xu and Wen-he Shen and Xi-yang Chen and Jian-ping Lin and Pei-lin Cen},
  journal={Biotechnology Letters},
  year={2005},
  volume={27},
  pages={1135-1140}
}
A novel high-throughput cultivation method was developed to rapidly screen large numbers of rapamycin-producing mutants of Streptomyces hygroscopicus by duplicate culturing of isolates on the surfaces of agar-solidified 96 wells in microtiter plates. One copy of the cultures was used for the rapamycin bioassay and the other identical copy, representing potentially high yielding strains, was preserved for further study. By integrating 96-well solid cultivation and the bioassay, we screened more… CONTINUE READING

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New process control strategy used in a rapamycin fermentation

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  • Proc. Biochem
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