A High-Throughput Assay for siRNA-Based Circadian Screens in Human U2OS Cells

@article{Vollmers2008AHA,
  title={A High-Throughput Assay for siRNA-Based Circadian Screens in Human U2OS Cells},
  author={Christopher Vollmers and Satchidananda Panda and Luciano DiTacchio},
  journal={PLoS ONE},
  year={2008},
  volume={3},
  pages={329 - 335}
}
The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells. 

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