A HPLC-MS method for the simultaneous quantification of fourteen antiretroviral agents in peripheral blood mononuclear cell of HIV infected patients optimized using medium corpuscular volume evaluation.

@article{DAvolio2011AHM,
  title={A HPLC-MS method for the simultaneous quantification of fourteen antiretroviral agents in peripheral blood mononuclear cell of HIV infected patients optimized using medium corpuscular volume evaluation.},
  author={Antonio D'Avolio and M. Simiele and Marco Siccardi and Lorena Baietto and Mauro Sciandra and Valentina Oddone and Francesca Romana Stefani and Silvia Agati and Jessica Cusato and Stefano Bonora and Giovanni di Perri},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2011},
  volume={54 4},
  pages={779-88}
}
A sensitive and accurate high performance liquid chromatography-mass spectrometric (HPLC-MS) method for the intracellular determination of 14 antiretroviral drugs in peripheral blood mononuclear cells (PBMCs) for HIV+ patients was validated. PBMCs are isolated by Ficoll density gradient centrifugation and cells count and the relative mean volume is performed with a Coulter(®) instrument. Extraction of drugs from PBMCs pellets was obtained with methanol:water (70:30, v/v), with quinoxaline added… CONTINUE READING