A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in Detergents.

@article{Yang2016AGT,
  title={A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in Detergents.},
  author={Zhengrong Yang and Christie G. Brouillette},
  journal={Methods in enzymology},
  year={2016},
  volume={567},
  pages={319-58}
}
Differential scanning calorimetry (DSC) detects protein thermal unfolding by directly measuring the heat absorbed. Simple DSC experiments that require relatively small amounts of pure material can provide a wealth of information related to structure, especially with respect to domain architecture, without the need for a complete thermodynamic analysis. Thus, DSC is an ideal additional tool for membrane protein characterization and also offers several advantages over indirect thermal unfolding… CONTINUE READING