A Giant Virus in Amoebae

  title={A Giant Virus in Amoebae},
  author={Bernard La Scola and St{\'e}phane Audic and Catherine Robert and Liang Jungang and Xavier de Lamballerie and Michel Drancourt and Richard J. Birtles and Jean-Michel Claverie and Didier Raoult},
  pages={2033 - 2033}
During a study following a pneumonia outbreak in 1992, a microorganism growing in amoebae and resembling a small Gram-positive coccus ([Fig. 1][1]A) was isolated from the water of a cooling tower in Bradford, England. Despite attempts with various extraction protocols and low-stringency polymerase 
Mimivirus in Pneumonia Patients
Mimivirus DNA was found in respiratory samples of patients with hospital-acquired pneumonia, and was used as an antigen in a migration inhibition factor assay, which indicated seroconversion in patients with both community- and hospital- acquired pneumonia.
Amoebae as Battlefields for Bacteria, Giant Viruses, and Virophages
It is shown that the virophage Sputnik 1, by reducing APM fitness, preserved BABL1 growth in acute and chronic models, highlighting the competition that occurs between them during natural host infection.
Screening Pneumonia Patients for Mimivirus
Acanthamoeba polyphaga mimivirus was not detected in any specimen, which suggests it is not a common respiratory pathogen.
Isolation of Giant Viruses of Acanthamoeba castellanii
The methodologic procedures regarding the prospecting and isolating giant viruses in A. castellanii are described, including the preparation of environmental samples, the culture of amoebas, and the observation of cytopathic effects that can indicate the presence and potential isolation of giant viruses.
Giant Viruses from Amoeba in a Post-Darwinist Viral World
This special issue of Intervirology is devoted to Mimivirus, the giant virus of amoebae, which is almost ubiquitous and found in the majority of unfiltered databank samples.
The role of giant viruses of amoebas in humans.
Isolation and identification of amoeba-resisting bacteria from water in human environment by using an Acanthamoeba polyphaga co-culture procedure.
Preliminary work demonstrates that the water environment in the vicinity of humans is a reservoir of ARB, including well-known pathogens for which amoebae and/or water was not recognized earlier as a possible reservoir.
Amoebal pathogens as emerging causal agents of pneumonia.
The current evidence for the emerging pathogenic role of various amoebae-resisting microorganisms as agents of RTIs in humans is reviewed and Legionella-likeAmoebal pathogens, novel Chlamydiae, waterborne mycobacteria and Bradyrhizobiaceae are discussed.
The discovery and characterization of Mimivirus, the largest known virus and putative pneumonia agent.
A virus, now named "Mimivirus," has been implicated as an agent of pneumonia in humans and, thus, should be considered a putative emerging pathogen.


Isolation and characterization of two viruses with large genome size infecting Chrysochromulina ericina (Prymnesiophyceae) and Pyramimonas orientalis (Prasinophyceae).
Two lytic viruses specific for Chrysochromulina ericina and Pyramimonas orientalis were isolated from Norwegian coastal waters in June 1998 and have several properties in common with other viruses infecting microalgae, suggesting that they belong to the Phycodnaviridae.
Common Origin of Four Diverse Families of Large Eukaryotic DNA Viruses
The conservation of the disulfide-oxidoreductase, a major capsid protein, and two virion membrane proteins indicates that the odd-shaped virions of poxviruses have evolved from the more common icosahedral virion seen in asfarviruses, iridoviruses, and phycodnaviruses.
Two‐dimensional motion of DNA bands during 120° pulsed‐field gel electrophoresis. I. Effect of molecular weight
The instantaneous position and velocity of bands of linear, double‐stranded DNA were measured during 120° pulsed‐field electrophoresis in 1% agarose gels, using a video micrometer capable of
Base-calling of automated sequencer traces using phred. I. Accuracy assessment.
The availability of massive amounts of DNA sequence information has begun to revolutionize the practice of biology. As a result, current large-scale sequencing output, while impressive, is not