A Gene Essential for De Novo Methylation and Development in Ascobolus Reveals a Novel Type of Eukaryotic DNA Methyltransferase Structure

@article{Malagnac1997AGE,
  title={A Gene Essential for De Novo Methylation and Development in Ascobolus Reveals a Novel Type of Eukaryotic DNA Methyltransferase Structure},
  author={F. Malagnac and B. Wendel and C. Goyon and G. Faugeron and D. Zickler and J. Rossignol and M. Noyer-Weidner and P. Vollmayr and T. Trautner and J. Walter},
  journal={Cell},
  year={1997},
  volume={91},
  pages={281-290}
}
  • F. Malagnac, B. Wendel, +7 authors J. Walter
  • Published 1997
  • Biology, Medicine
  • Cell
  • Molecular mechanisms determining methylation patterns in eukaryotic genomes still remain unresolved. We have characterized, in Ascobolus, a gene for de novo methylation. This novel eukaryotic gene, masc1, encodes a protein that has all motifs of the catalytic domain of eukaryotic C5-DNA-methyltransferases but is unique in that it lacks a regulatory N-terminal domain. The disruption of masc1 has no effect on viability or methylation maintenance but prevents the de novo methylation of DNA repeats… CONTINUE READING
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