A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis.

@article{Schlotter2009AGA,
  title={A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis.},
  author={Yvette M. Schlotter and Eveline Z Veenhof and Bas Brinkhof and V P M G Rutten and Bart Spee and Ton Willemse and Louis C. Penning},
  journal={Veterinary immunology and immunopathology},
  year={2009},
  volume={129 1-2},
  pages={115-8}
}
Quantitative real time PCR (Q-PCR) is the method of choice to study mRNA expression levels. Since Q-PCR is very sensitive, normalization of the data with stably expressed reference genes if of utmost importance. The stability of reference genes depends on the tissue and the species of interest. Therefore, evaluation of the stability of reference genes must be performed for each new tissue and species under study. The stability of B2M, GAPDH, HPRT, SRPR, hnRNPH, GUSB, RPL8, RPS5, and RPS19 was… CONTINUE READING