A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta[w]

@article{Curtis2003AGC,
  title={A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta[w]},
  author={M. D. Curtis and U. Grossniklaus},
  journal={Plant Physiology},
  year={2003},
  volume={133},
  pages={462 - 469}
}
  • M. D. Curtis, U. Grossniklaus
  • Published 2003
  • Biology, Medicine
  • Plant Physiology
  • The current challenge, now that two plant genomes have been sequenced, is to assign a function to the increasing number of predicted genes. In Arabidopsis, approximately 55% of genes can be assigned a putative function, however, less than 8% of these have been assigned a function by direct experimental evidence. To identify these functions, many genes will have to undergo comprehensive analyses, which will include the production of chimeric transgenes for constitutive or inducible ectopic… CONTINUE READING
    2,196 Citations
    A Versatile Set of Ligation-Independent Cloning Vectors for Functional Studies in Plants1[C][W][OA]
    • 93
    • PDF
    A modified Gateway cloning strategy for overexpressing tagged proteins in plants
    • 60
    A Series of TA-Based and Zero-Background Vectors for Plant Functional Genomics
    • 16
    • PDF
    Gateway-compatible vectors for plant functional genomics and proteomics.
    • 1,436
    • PDF
    Gateway-compatible inducible vector set for the functional analysis of transcription factors in plants
    Rapid cloning of genes and promoters for functional analyses.
    • P. Schenk
    • Biology, Medicine
    • Methods in molecular biology
    • 2014
    • 2
    A Golden Gate and Gateway double-compatible vector system for high throughput functional analysis of genes.
    A Versatile Zero Background T-Vector System for Gene Cloning and Functional Genomics1[C][W][OA]
    • 281
    • PDF

    References

    SHOWING 1-10 OF 31 REFERENCES
    DNA cloning using in vitro site-specific recombination.
    • 1,000
    • PDF
    Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
    • 1,295
    GATEWAY vectors for Agrobacterium-mediated plant transformation.
    • 2,985
    GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    • 8,901
    • PDF
    Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants.
    • 237