A GH57 Family Amylopullulanase from Deep-Sea Thermococcus siculi: Expression of the Gene and Characterization of the Recombinant Enzyme

@article{Jiao2010AGF,
  title={A GH57 Family Amylopullulanase from Deep-Sea Thermococcus siculi: Expression of the Gene and Characterization of the Recombinant Enzyme},
  author={Yu-Liang Jiao and Shujun Wang and Ming-Sheng Lv and Jin-Li Xu and Yao-Wei Fang and Shu Zheng Liu},
  journal={Current Microbiology},
  year={2010},
  volume={62},
  pages={222-228}
}
The gene encoding a new extracellular amylopullulanase (type II pullulanase) was cloned from an extremely thermophilic anaerobic archaeon Thermococcus siculi strain HJ21 isolated previously from a deep-sea hydrothermal vent. The functional hydrolytic domain of the amylopullulanase (TsiApuN) and its MalE fusion protein (MTsiApuN) were expressed heterologously. The complete amylopullulanase (TsiApu) was also purified from fermentation broth of the strain. The pullulanase and amylase activities of… CONTINUE READING

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