A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg(II) in environment.

@article{Priyadarshi2012AGB,
  title={A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg(II) in environment.},
  author={Himanshu Priyadarshi and Absar Alam and Pathakota Gireesh-Babu and Rekha Das and Pankaj Kishore and Shivendra Kumar and Aparna Chaudhari},
  journal={Journal of environmental sciences},
  year={2012},
  volume={24 5},
  pages={963-8}
}
A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of lambda phage. The genetic elements used include a regulatory protein gene (merR) along with operator/promoter (O/P) derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the… CONTINUE READING

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