A GAL4-based targeted activation tagging system in Arabidopsis thaliana.

  title={A GAL4-based targeted activation tagging system in Arabidopsis thaliana.},
  author={Takamitsu Waki and Shunsuke Miyashima and Miyako Nakanishi and Yoichi Ikeda and Takashi Hashimoto and Keiji Nakajima},
  journal={The Plant journal : for cell and molecular biology},
  volume={73 3},
Activation tagging is a powerful tool for discovering novel genes that are not easily identified by loss-of-function (lof) screening due to genetic redundancy or lethality. Although the current activation tagging system, which involves a viral enhancer sequence, has been used for a decade, alternative methods that allow organ- or tissue-specific activation are required to identify genes whose strong activation leads to loss of fertility or viability. Here, we established a GAL4/UAS activation… CONTINUE READING
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