A Fungal Sarcolemmal Membrane-Associated Protein (SLMAP) Homolog Plays a Fundamental Role in Development and Localizes to the Nuclear Envelope, Endoplasmic Reticulum, and Mitochondria

@article{Nordzieke2014AFS,
  title={A Fungal Sarcolemmal Membrane-Associated Protein (SLMAP) Homolog Plays a Fundamental Role in Development and Localizes to the Nuclear Envelope, Endoplasmic Reticulum, and Mitochondria},
  author={Steffen Nordzieke and Thomas Zobel and Benjamin Fr{\"a}nzel and Dirk Wolters and Ulrich K{\"u}ck and Ines Teichert},
  journal={Eukaryotic Cell},
  year={2014},
  volume={14},
  pages={345 - 358}
}
ABSTRACT Sarcolemmal membrane-associated protein (SLMAP) is a tail-anchored protein involved in fundamental cellular processes, such as myoblast fusion, cell cycle progression, and chromosomal inheritance. Further, SLMAP misexpression is associated with endothelial dysfunctions in diabetes and cancer. SLMAP is part of the conserved striatin-interacting phosphatase and kinase (STRIPAK) complex required for specific signaling pathways in yeasts, filamentous fungi, insects, and mammals. In… 
Fsr1, a striatin homologue, forms an endomembrane-associated complex that regulates virulence in the maize pathogen Fusarium verticillioides.
TLDR
Several novel components in the STRIPAK complex that regulates F. verticillioides stalk rot virulence are identified, and it is proposed that the correct organization and localization of Fsr1 are critical for STRIPak complex function.
Assembly of a heptameric STRIPAK complex is required for coordination of light-dependent multicellular fungal development with secondary metabolism in Aspergillus nidulans
TLDR
The discovery of six striatin (StrA) interacting proteins (Sips), which form a heptameric complex in the filamentous fungus Aspergillus nidulans, strengthens the hypothesis that mammals and fungi use similar proteins for their immune response and secondary metabolite production, respectively.
A Hippo Pathway-Related GCK Controls Both Sexual and Vegetative Developmental Processes in the Fungus Sordaria macrospora
TLDR
Compelling evidence is provided that SmKIN3 is involved in fruiting body formation, hyphal fusion, and septation in filamentous fungi and links both the SIN and STRIPAK complex, thereby regulating multiple key cellular processes.
Germinal Center Kinases SmKIN3 and SmKIN24 Are Associated with the Sordaria macrospora Striatin-Interacting Phosphatase and Kinase (STRIPAK) Complex
TLDR
The presence and function of STRIPAK-associated kinases in ascomycetes are reported on and it is found that both kinases were present at the septa and deletion of both Smkin3 and Smkin24 led to abnormal septum distribution.
Analysis of the Putative Nucleoporin POM33 in the Filamentous Fungus Sordaria macrospora
TLDR
It cannot be excluded that SmPOM33 is involved in temporarily anchoring the SmSTRIPAK to the NE or other sites in the cell, and ER-membrane proteins involved in ER morphology, protein translocation, glycosylation, sterol biosynthesis and Ca2+-transport were significantly enriched.
Deletion of Smgpi1 encoding a GPI‐anchored protein suppresses sterility of the STRIPAK mutant ΔSmmob3 in the filamentous ascomycete Sordaria macrospora
TLDR
The interplay of the glycosylphosphatidylinositol‐anchored protein SmGPI1 with the kinase activator SmMOB3, a core component of human and fungal STRIPAK complexes, is reported on.
The STRIPAK component SipC is involved in morphology and cell-fate determination in the nematode-trapping fungus Duddingtonia flagrans
TLDR
The role of the STRIPAK complex is analyzed in the nematode-trapping fungus Duddingtonia flagrans which forms three-dimensional, adhesive trapping networks to capture Caenorhabditis elegans and shows that lack of theSTRIPAK component SipC results in incomplete loop formation and column-like trap structures with elongated compartments.
The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes
TLDR
An extended global proteome and phosphoproteome study using the wild type as well as STRIPAK single and double deletion mutants from the filamentous fungus Sordaria macrospora suggests that differential phosphorylation of GUL1 regulates developmental processes such as fruiting body maturation and hyphal morphogenesis.
Catalytic Subunit 1 of Protein Phosphatase 2A Is a Subunit of the STRIPAK Complex and Governs Fungal Sexual Development
TLDR
Data indicate links between the STRIPAK complex and other developmental signaling pathways, implying the presence of a large interconnected signaling network that controls eukaryotic developmental processes.
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