A Fluorescent, Reagentless Biosensor for ADP Based on Tetramethylrhodamine-Labeled ParM

@inproceedings{Kunzelmann2010AFR,
  title={A Fluorescent, Reagentless Biosensor for ADP Based on Tetramethylrhodamine-Labeled ParM},
  author={Simone Kunzelmann and Martin R Webb},
  booktitle={ACS chemical biology},
  year={2010}
}
Fluorescence assays for ADP detection are of considerable current interest, both in basic research and in drug discovery, as they provide a generic method for measuring the activity of ATPases and kinases. The development of a novel fluorescent biosensor is described that is based on a tetramethylrhodamine-labeled, bacterial actin homologue, ParM. The design of the biosensor takes advantage of the large conformational change of ParM on ADP binding and the strong quenching of the… CONTINUE READING
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