A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants.

@article{AliyevaSchnorr2015AFA,
  title={A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants.},
  author={Lala Aliyeva-Schnorr and Lu Ma and Andreas Houben},
  journal={Journal of visualized experiments : JoVE},
  year={2015},
  volume={106},
  pages={e53470}
}
Preparation of chromosome spreads is a prerequisite for the successful performance of fluorescence in situ hybridization (FISH). Preparation of high quality plant chromosome spreads is challenging due to the rigid cell wall. One of the approved methods for the preparation of plant chromosomes is a so-called drop preparation, also known as drop-spreading or air-drying technique. Here, we present a protocol for the fast preparation of mitotic chromosome spreads suitable for the FISH detection of… CONTINUE READING