A FRET probe for cell-based imaging of ganglioside-processing enzyme activity and high-throughput screening.

@article{Yang2015AFP,
  title={A FRET probe for cell-based imaging of ganglioside-processing enzyme activity and high-throughput screening.},
  author={Guanghua Yang and Caishun Li and Michael A. Fischer and Christopher W. Cairo and Yan Feng and Stephen G Withers},
  journal={Angewandte Chemie},
  year={2015},
  volume={54 18},
  pages={
          5389-93
        }
}
Gangliosides are important signaling molecules in the cell membrane and are processed by several enzymes. Deficiencies in these enzymes can cause human lysosomal storage diseases. Building an understanding of the pathways of glycosphingolipid catabolism requires methods for the analysis of these enzymatic activities A GM3-derived FRET probe was synthesized chemoenzymatically for the detection and quantitation of a range of ganglioside-degrading enzymes, both in cell lysates and in living cells… CONTINUE READING
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