A FERM domain autoregulates Drosophila myosin 7a activity.

  title={A FERM domain autoregulates Drosophila myosin 7a activity.},
  author={Yi Yang and Thomas G Baboolal and Verl B Siththanandan and Michael Isaac Chen and Matthew L. Walker and P. J. Knight and Michelle Peckham and James R Sellers},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={106 11},
Full-length Drosophila myosin 7a (myosin 7a-FL) has a complex tail containing a short predicted coiled coil followed by a MyTH4-FERM domain, an SH3 domain, and a C-terminal MyTH4-FERM domain. Myosin 7a-FL expressed in Sf9 cells is monomeric despite the predicted coiled coil. We showed previously that Subfragment-1 (S1) from this myosin has MgATPase of V(max) approximately 1 s(-1) and K(ATPase) approximately 1 microM actin. We find that myosin 7a-FL has V(max) similar to S1 but K(ATPase… CONTINUE READING