A Double-MonoclonallmmunoenzymometncAssay for AIpha 1-Fetoprotein Modified for Increased AnalyticalPrecision

Abstract

We modified a one-step, two-site, double monoclonal immunoenzymometric assay (Abbott Laboratories) for serum alpha1-fetoprotein (AFP) to increase its sensitivity and improve test precision at the low end. We increased sample size, incubation interval, and reaction time and temperature, and decreased the final reaction volume. Interassay CVs for the modified… (More)

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