A Distinct Proof on Interplay between Trehalose and Guanidinium Chloride for the Stability of Stem Bromelain.

@article{Rani2016ADP,
  title={A Distinct Proof on Interplay between Trehalose and Guanidinium Chloride for the Stability of Stem Bromelain.},
  author={A Pramela Rani and Pannuru Venkatesu},
  journal={The journal of physical chemistry. B},
  year={2016},
  volume={120 34},
  pages={8863-72}
}
Guanidinium chloride (GdnHCl), a potential denaturant, is well-known to denature a number of proteins in vitro as well as in vivo studies. Its deleterious action on stem bromelain (BM) is quite prominent resulting decrease in protein structure and stability. The counteraction of this adverse effect of GdnHCl by the use of osmolytes is scarcely studied and the mechanism is still illusive and not exclusive. For the first time, to test elegant and simple counteraction hypothesis as a general… CONTINUE READING