A Discrete Class of Intergenic DNA Dictates Meiotic DNA Break Hotspots in Fission Yeast

@article{Cromie2007ADC,
  title={A Discrete Class of Intergenic DNA Dictates Meiotic DNA Break Hotspots in Fission Yeast},
  author={Gareth A Cromie and Randy W. Hyppa and Hugh P Cam and Joseph A Farah and Shiv I S Grewal and Gerald R Smith},
  journal={PLoS Genetics},
  year={2007},
  volume={3},
  pages={87 - 90}
}
Meiotic recombination is initiated by DNA double-strand breaks (DSBs) made by Spo11 (Rec12 in fission yeast), which becomes covalently linked to the DSB ends. Like recombination events, DSBs occur at hotspots in the genome, but the genetic factors responsible for most hotspots have remained elusive. Here we describe in fission yeast the genome-wide distribution of meiosis-specific Rec12-DNA linkages, which closely parallel DSBs measured by conventional Southern blot hybridization. Prominent DSB… CONTINUE READING