A Direct Method to Isolate DNA from Phyllosphere Microbial Communities without Disrupting Leaf Tissues.

@article{Suda2008ADM,
  title={A Direct Method to Isolate DNA from Phyllosphere Microbial Communities without Disrupting Leaf Tissues.},
  author={Wataru Suda and Michiei Oto and Seigo Amachi and Hirofumi Shinoyama and Masahiro Shishido},
  journal={Microbes and environments},
  year={2008},
  volume={23 3},
  pages={248-52}
}
We developed a method for direct DNA isolation from phyllosphere microbial communities, designated Direct-DIP. This method comprises DNA extraction from non-shredded leaves with benzyl chloride, and DNA purification by gel filtration. Scanning electron microscopy showed that epiphytic microorganisms were completely removed from the leaf surface after benzyl chloride treatment, while microstructures of the leaf were not damaged. Clear DGGE profiles were obtained regardless of the plant species… CONTINUE READING
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