A DNA sequence-specific electrochemical biosensor based on alginic acid-coated cobalt magnetic beads for the detection of E. coli.

@article{Geng2011ADS,
  title={A DNA sequence-specific electrochemical biosensor based on alginic acid-coated cobalt magnetic beads for the detection of E. coli.},
  author={Ping Geng and Xinai Zhang and Yingqiao Teng and Ying Fu and Lili Xu and Min Xu and Litong Jin and Wen Zhang},
  journal={Biosensors & bioelectronics},
  year={2011},
  volume={26 7},
  pages={3325-30}
}
A new type of DNA sequence-specific electrochemical biosensor based on magnetic beads for the detection of Escherichia coli is reported in the present work. Alginic acid-coated cobalt magnetic beads, capped with 5'-(NH(2)) oligonucleotide and employed not only for magnetic separation but also as the solid adsorbent, were used as DNA probes to hybridize with the target E. coli DNA sequence. This assay was specific for E. coli detection depending on the uid A gene, which encodes for the enzyme… CONTINUE READING

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