A DNA extraction method for RAPD analysis from plants rich in soluble polysaccharides

Abstract

A simple procedure for the isolation of DNA from mature leaf tissue was developed. This procedure purified DNA using Sephacryl S-1000 column and PEG 8000 precipitation. Polysaccharide-like components were successfully removed from DNA samples from species in which polysaccharides were found to be difficult to remove by phenol/chloroform extraction. The DNA… (More)
DOI: 10.1007/BF02668744

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