A DNA enzyme with Mg(2+)-dependent RNA phosphoesterase activity.

  title={A DNA enzyme with Mg(2+)-dependent RNA phosphoesterase activity.},
  author={Ronald R. Breaker and Gerald F Joyce},
  journal={Chemistry & biology},
  volume={2 10},
BACKGROUND Previously we demonstrated that DNA can act as an enzyme in the Pb(2+)-dependent cleavage of an RNA phosphoester. This is a facile reaction, with an uncatalyzed rate for a typical RNA phosphoester of approximately 10(-4) min-1 in the presence of 1 mM Pb(OAc)2 at pH 7.0 and 23 degrees C. The Mg(2+)-dependent reaction is more difficult, with an uncatalyzed rate of approximately 10(-7) min-1 under comparable conditions. Mg(2+)-dependent cleavage has special relevance to biology because… CONTINUE READING

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