A Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus with Efficient Gene Transfer and Expression

@article{Li2000ACR,
  title={A Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus with Efficient Gene Transfer and Expression},
  author={Hai-ou Li and Yafeng Zhu and Makoto Asakawa and Hidekazu Kuma and Takahiro Hirata and Yasuji Ueda and Yun-Sik Lee and Masayuki Fukumura and Akihiro Iida and Atsushi Kato and Yoshiyuki Nagai and Mamoru Hasegawa},
  journal={Journal of Virology},
  year={2000},
  volume={74},
  pages={6564 - 6569}
}
ABSTRACT We have recovered a virion from defective cDNA of Sendai virus (SeV) that is capable of self-replication but incapable of transmissible-virion production. This virion delivers and expresses foreign genes in infected cells, and this is the first report of a gene expression vector derived from a defective viral genome of theParamyxoviridae. First, functional ribonucleoprotein complexes (RNPs) were recovered from SeV cloned cDNA defective in the F (envelope fusion protein) gene, in the… 
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Rescue of Sendai Virus from Cloned cDNA.
TLDR
Reverse genetics system of SeV is relatively efficient compared to other paramyxoviruses, but alternative approaches to rescue poorly growing mutant viruses are also available.
A New Sendai Virus Vector Deficient in the Matrix Gene Does Not Form Virus Particles and Shows Extensive Cell-to-Cell Spreading
TLDR
SeV/ΔM is a novel type of vector with the characteristic features of loss of virus particle formation and gain of cell-to-cell spreading via a mechanism dependent on the activation of the fusion protein.
Recombinant Sendai viruses expressing different levels of a foreign reporter gene.
Characterization of P gene-deficient rabies virus: propagation, pathogenicity and antigenicity.
An improved method for recovery of F-defective Sendai virus expressing foreign genes from cloned cDNA.
Sendai virus, an RNA virus with no risk of genomic integration, delivers CRISPR/Cas9 for efficient gene editing
TLDR
Sendai virus is repurpose, an RNA virus with no viral DNA phase and that replicates solely in the cytoplasm, as a delivery system for efficient Cas9-mediated gene editing and opens the door to using Sendai virus as well as other related paramyxoviruses as versatile and efficient tools for gene editing.
Generation and characterization of P gene-deficient rabies virus.
A defective nontransmissible recombinant Sendai virus mediates efficient gene transfer to airway epithelium in vivo
TLDR
The development of an F-defective nontransmissible SeV, which can still efficiently mediate gene transfer to the airway epithelium, represents the first important step towards the use of a cytoplasmic RNA viral vector in clinical trials of gene therapy.
Pseudotyped Lentivirus Vectors Derived from Simian Immunodeficiency Virus SIVagm with Envelope Glycoproteins from Paramyxovirus
TLDR
The mutant SeV F- and HN-pseudotyped SIVagm vector was able to transduce various types of animal and human cell lines and have considerable potential for effective use in gene therapy for various therapies, including respiratory diseases.
A decade after the generation of a negative-sense RNA virus from cloned cDNA - what have we learned?
TLDR
R reverse genetics, or the de novo synthesis of negative-sense RNA viruses from cloned cDNA, has become a reliable laboratory method that can be used to study this large group of medically and economically important viruses.
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