ArsR (or ArsR/SmtB) family metalloregulatory homodimeric repressors collectively respond to a wide range of metal ion inducers in regulating homeostasis and resistance of essential and nonessential metal ions in bacteria. BxmR from the cyanobacterium Osciliatoria brevis is the first characterized ArsR protein that senses both Cu (I)/Ag (I) and divalent… (More)
FIGURE 6: 111Ag and 111mCd perturbed angular correlation (PAC) spectroscopy of AgI-substituted and CdII-substituted wild-type (WT) BxmR, respectively. Top (panels A and B, 111Ag PAC; panel C, 111mCd PAC): the experimentally determined perturbation function (with error bars, in blue) and fit (boldfaced black line). Bottom (panels D-F): Fourier transformations of the experimental perturbation function in panels A-C, respectively (thin line) and fit (boldfaced line). The data from 111Ag PAC experiments in panels A and B are identical and merely show the results of two independent fits (1 and 2; see Table 2); these are multiplied by -1 to allow for easy comparison with 111mCd PAC data (panel C). Parameters that derive from the fitted curves are compiled in Table 2.