A Cryptic Targeting Signal Creates a Mitochondrial FEN1 Isoform with Tailed R-Loop Binding Properties

@inproceedings{Kazak2013ACT,
  title={A Cryptic Targeting Signal Creates a Mitochondrial FEN1 Isoform with Tailed R-Loop Binding Properties},
  author={Lawrence Kazak and Aurelio de los Reyes and Jiuya He and Stuart R. Wood and Gloria Brea-Calvo and Torgeir T. Holen and Ian Holt},
  booktitle={PloS one},
  year={2013}
}
A growing number of DNA transacting proteins is found in the nucleus and in mitochondria, including the DNA repair and replication protein Flap endonuclease 1, FEN1. Here we show a truncated FEN1 isoform is generated by alternative translation initiation, exposing a mitochondrial targeting signal. The shortened form of FEN1, which we term FENMIT, localizes to mitochondria, based on import into isolated organelles, immunocytochemistry and subcellular fractionation. In vitro FENMIT binds to flap… CONTINUE READING