A Crosslinking Analysis of GAP-43 Interactions with Other Proteins in Differentiated N1E-115 Cells

@article{Ollom2008ACA,
  title={A Crosslinking Analysis of GAP-43 Interactions with Other Proteins in Differentiated N1E-115 Cells},
  author={Callise M. Ollom and John B. Denny},
  journal={International Journal of Molecular Sciences},
  year={2008},
  volume={9},
  pages={1753 - 1771}
}
It has been suggested that GAP-43 (growth-associated protein) binds to various proteins in growing neurons as part of its mechanism of action. To test this hypothesis in vivo, differentiated N1E-115 neuroblastoma cells were labeled with [(35)S]-amino acids and were treated with a cleavable crosslinking reagent. The cells were lysed in detergent and the lysates were centrifuged at 100,000 x g to isolate crosslinked complexes. Following cleavage of the crosslinks and analysis by two-dimensional… CONTINUE READING