A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli.

@article{Jger1997ACG,
  title={A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli.},
  author={Wolfgang J{\"a}ger and J{\"o}rn Kalinowski and Alfred P{\"u}hler},
  journal={Journal of bacteriology},
  year={1997},
  volume={179 7},
  pages={2449-51}
}
A chromosomal DNA fragment from the erythromycin-sensitive bacterium Corynebacterium glutamicum ATCC 13032 was shown to mediate resistance against erythromycin, tetracycline, puromycin, and bleomycin in Escherichia coli. Multicopy cloning of the fragment did not cause a resistance phenotype in C. glutamicum. The corresponding gene encodes a hydrophobic protein with 12 potential transmembrane-spanning ex-helical segments showing similarity to drug-H+ antiporters.