A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides

  title={A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides},
  author={Wei Wang and Nan Wang and Y. Zhang and Z. Cai and Qihe Chen and Guo-qing He},
  journal={ISRN Biotechnology},
  • Wei Wang, Nan Wang, +3 authors Guo-qing He
  • Published 2013
  • Chemistry, Medicine
  • ISRN Biotechnology
  • A convenient and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for angiotensin I-converting enzyme inhibitory peptides assay was described in this paper. The mobile phase consisted of 70% A (0.05% TFA and 0.05% triethylamine in water, pH = 2.9–3.3) and 30% B (100% acetonitrile) using an Isogradient program. The flow rate was 0.5 mL/min. The absorb wavelength was 226.5 nm; the column temperature was controlled at 25°C. This method for angiotensin I-converting… CONTINUE READING
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