A Connectome and Analysis of the Adult Drosophila Central Brain

@article{Scheffer2020ACA,
  title={A Connectome and Analysis of the Adult Drosophila Central Brain},
  author={Louis K. Scheffer and C. Xu and M. Januszewski and Zhiyuan Lu and Shin-ya Takemura and K. Hayworth and Gary B. Huang and K. Shinomiya and Jeremy B. Maitin-Shepard and Stuart E. Berg and Jody Clements and P. Hubbard and W. Katz and L. Umayam and Ting Zhao and David Ackerman and T. Blakely and J. Bogovic and Tom Dolafi and Dagmar Kainmueller and Takashi Kawase and K. Khairy and Laramie Leavitt and Peter H. Li and Larry Lindsey and Nicole L. Neubarth and D. J. Olbris and H. Otsuna and Eric T. Trautman and Masayoshi Ito and Jens Goldammer and T. Wolff and R. Svirskas and P. Schlegel and E. Neace and Christopher J Knecht and Chelsea X. Alvarado and Dennis A Bailey and Samantha Ballinger and J. Borycz and Brandon S Canino and Natasha Cheatham and Michael Cook and M. Dreher and Octave Duclos and Bryon Eubanks and K. Fairbanks and Samantha Finley and N. Forknall and Audrey Francis and G. Hopkins and Emily M Joyce and SungJin Kim and Nicole A. Kirk and Julie Kovalyak and Shirley Lauchie and Alanna Lohff and Charli Maldonado and Emily A. Manley and Sari McLin and Caroline Mooney and Miatta Ndama and Omotara Ogundeyi and Nneoma Okeoma and Christopher Ordish and Nicholas Padilla and Christopher M Patrick and Tyler Paterson and Elliott E Phillips and E. M. Phillips and Neha Rampally and Caitlin Ribeiro and Madelaine K Robertson and J. Rymer and S. Ryan and Megan Sammons and Anne K. Scott and Ashley L. Scott and A. Shinomiya and Claire Smith and Kelsey Smith and Natalie L Smith and Margaret A. Sobeski and Alia Suleiman and Jackie Swift and Satoko Takemura and Iris Talebi and Dorota Tarnogorska and Emily Tenshaw and Temour Tokhi and J. Walsh and Tansy Yang and J. Horne and F. Li and Ruchi Parekh and P. Rivlin and V. Jayaraman and Kei Ito and S. Saalfeld and R. George and I. Meinertzhagen and G. Rubin and H. Hess and Viren Jain and Stephen M. Plaza},
  journal={bioRxiv},
  year={2020}
}
The neural circuits responsible for animal behavior remain largely unknown. We summarize new methods and present the circuitry of a large fraction of the brain of the fruit fly Drosophila melanogaster. Improved methods include new procedures to prepare, image, align, segment, find synapses in, and proofread such large data sets. We define cell types, refine computational compartments, and provide an exhaustive atlas of cell examples and types, many of them novel. We provide detailed circuits… Expand
The connectome of the adult Drosophila mushroom body: implications for function
TLDR
This work identifies new components of the MB circuit in Drosophila, including extensive visual input and MB output neurons (MBONs) with direct connections to descending neurons, and provides insights into the circuitry used to integrate MB outputs, connectivity between the MB and the central complex and inputs to DANs, including feedback from MBONs. Expand
Evidence of wiring development processes from the connectome of adult Drosophila
TLDR
Support is found for the hypothesis that surface proteins specific to cell types determine the directivity of connections and evidence suggests that at least some of this distinction is post-synaptic. Expand
Graph Properties of the Adult Drosophila Central Brain
TLDR
This work considers the Drosophila central brain connectome as a directed graph with weighted edges, which enables it to look at a number of graph properties, compare them to human designed logic systems, and speculate on how this may affect function. Expand
Decoding gene regulation in the fly brain
The Drosophila brain is a work horse in neuroscience. Single-cell transcriptome analysis 1–5, 3D morphological classification 6, and detailed EM mapping of the connectome 7–10 have revealed anExpand
A distributed dopamine-gated circuit underpins reproductive state-dependent behavior in Drosophila females
TLDR
The data in the fly suggests that reproductive state-dependent expression of female choice behavior is regulated by a dopamine-gated distributed learning circuit comprising both higher olfactory brain centers. Expand
Information flow, cell types and stereotypy in a full olfactory connectome
TLDR
A generally applicable strategy to extract information flow and layered organisation from connectome graphs, mapping olfactory input to descending interneurons is developed, identifying a range of motifs including highly lateralised circuits in the antennal lobe and patterns of convergence downstream of the mushroom body and lateral horn. Expand
Reconstruction of motor control circuits in adult Drosophila using automated transmission electron microscopy
TLDR
This work presents a synapse-resolution EM dataset containing the ventral nerve cord of an adult female Drosophila melanogaster, and shows that a specific class of leg sensory neurons directly synapse onto the largest-caliber motor neuron axons on both sides of the body, representing a unique feedback pathway for fast limb control. Expand
Connectomic features underlying diverse synaptic connection strengths and subcellular computation
Connectomes generated from electron microscopy images of neural tissue unveil the complex morphology of every neuron and the locations of every synapse interconnecting them. These wiring diagrams mayExpand
The connectome predicts resting state functional connectivity across the Drosophila brain
TLDR
It is found that functional connectivity is strongly associated with the strength of both direct and indirect anatomical pathways, and some brain regions show considerably higher functional connectivity than is predicted based on their direct anatomical connections. Expand
The number of neurons in Drosophila and mosquito brains
TLDR
This study provides experimental evidence for the total number of neurons in Drosophila and mosquito brains and identifies obvious sexual dimorphism in the neuronal and non-neuronal cell population of these insects. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 127 REFERENCES
A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster
TLDR
Recon reconstructions of the entire brain of an adult female fly show that this freely available EM volume supports mapping of brain-spanning circuits, which will significantly accelerate Drosophila neuroscience. Expand
Connectome studies on Drosophila: a short perspective on a tiny brain
TLDR
Attempts in Drosophila anticipate parallel attempts in other systems, notably the inner plexiform layer of the vertebrate retina, and augment the one complete connectome already available to us, that available for 30 years in the nematode Caenorhabditis elegans. Expand
Neuroarchitecture and neuroanatomy of the Drosophila central complex: A GAL4-based dissection of protocerebral bridge neurons and circuits
TLDR
An updated map of the Drosophila central complex will facilitate a deeper behavioral and physiological dissection of this sophisticated set of structures and provides new insights into the anatomical structure of the four components of the central complex and its accessory neuropils. Expand
Signal Propagation in Drosophila Central Neurons
TLDR
This work uses morphological and electrophysiological data to deduce the passive membrane properties of antennal lobe projection neurons and to build a compartmental model of their electrotonic structure, finding that these neurons are electrotonically extensive and that a somatic recording electrode can only imperfectly control the voltage in the rest of the cell. Expand
The functional organization of descending sensory-motor pathways in Drosophila
TLDR
The nerve cord of Drosophila melanogaster is a layered system of neuropils reflecting the fly’s capability for two largely independent means of locomotion -- walking and flight -- using distinct sets of appendages. Expand
Automatic Neuron Type Identification by Neurite Localization in the Drosophila Medulla
TLDR
This work introduces algorithms to cluster similarly-shaped neurons, where 3D neuronal shapes are represented as skeletons, and proposes a novel location-sensitive clustering algorithm that shows clustering results on neurons reconstructed from the Drosophila medulla that show high-accuracy. Expand
An unbiased template of the Drosophila brain and ventral nerve cord
TLDR
This work created high-resolution templates of the Drosophila brain and ventral nerve cord using the best-available technologies for imaging, artifact correction, stitching, and template construction using groupwise registration and demonstrates that theirs enables more accurate registration with fewer local deformations in shorter time. Expand
Automatic Detection of Synaptic Partners in a Whole-Brain Drosophila EM Dataset
TLDR
This work presents an automatic method for synaptic partner identification in insect brains, which uses convolutional neural networks to identify post- synapses sites and their pre-synaptic partners and requires only simple post-processing to obtain final predictions. Expand
Flybrain neuron database: A comprehensive database system of the Drosophila brain neurons
TLDR
The Flybrain NDB is constructed, which serves as a platform to collect and provide information about all the types of neurons published so far in the brain of Drosophila melanogaster, and the preferential connectivity among optic lobe layers and the plausible olfactory sensory map in the lateral horn is analyzed to show the usefulness of such a database. Expand
Neuronal assemblies of the Drosophila mushroom body
  • Nobuaki K. Tanaka, H. Tanimoto, Kei Ito
  • Biology, Medicine
  • The Journal of comparative neurology
  • 2008
TLDR
The laminar arrangement of the Kenyon cell axons and segmented organization of the MBENs together divide the lobes into smaller synaptic units, possibly facilitating characteristic interaction between intrinsic and extrinsic neurons in each unit for different functional activities along the longitudinal lobe axes and between lobes. Expand
...
1
2
3
4
5
...