A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase

Abstract

The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed an in silico analysis of the specificity and coverage of 51 universal and 35 group-specific nifH primers… (More)
DOI: 10.1371/journal.pone.0042149

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