A Completely De Novo ATPase from Combinatorial Protein Design.

  title={A Completely De Novo ATPase from Combinatorial Protein Design.},
  author={Michael S. Wang and Michael H. Hecht},
  journal={Journal of the American Chemical Society},
Our understanding of biological chemistry is shaped by the observation that all life comes from other life - as Pasteur put it, omne vivum ex vivo. A key step in expanding our biochemical vocabulary is to recapitulate biogenic catalysis using non-natural sequences that did not arise from common ancestry. Here we describe an enzyme designed completely de novo that hydrolyses ATP. This protein was designed to lack β-sheet structure and is competitively inhibited by magnesium, two traits that are… 
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