A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.

  title={A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.},
  author={Thomas Treiber and Nora Treiber and Uwe Plessmann and Simone Harlander and Julia-Lisa Dai\ss and Norbert Eichner and Gerhard Lehmann and Kevin Schall and Henning Urlaub and Gunter Meister},
  journal={Molecular cell},
  volume={66 2},
During microRNA (miRNA) biogenesis, two endonucleolytic reactions convert stem-loop-structured precursors into mature miRNAs. These processing steps can be posttranscriptionally regulated by RNA-binding proteins (RBPs). Here, we have used a proteomics-based pull-down approach to map and characterize the interactome of a multitude of pre-miRNAs. We identify ∼180 RBPs that interact specifically with distinct pre-miRNAs. For functional validation, we combined RNAi and CRISPR/Cas-mediated knockout… CONTINUE READING