A Comparative Analysis of the Phosphorylation and Biochemical Properties of Wild Type and Host Range Variant DNA Binding Proteins of Human Adenovirus 5

@article{Harfst1999ACA,
  title={A Comparative Analysis of the Phosphorylation and Biochemical Properties of Wild Type and Host Range Variant DNA Binding Proteins of Human Adenovirus 5},
  author={Eva Harfst and Keith N Leppard},
  journal={Virus Genes},
  year={1999},
  volume={18},
  pages={97-106}
}
Specific mutation of the DNA binding protein (DBP) of human adenovirus types 2 and 5 can extend the host range of these viruses to simian cells. These mutations replace histidine at position 130 in the highly phosphorylated N-terminal domain of DBP with a potentially phophorylatable tyrosine residue. To investigate the possibility that alternative phosphorylation might contribute to the functional differences between wild type (wt) and host range (hr) DBP molecules, radiolabeled proteins were… CONTINUE READING
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