A Combined Screening Platform for HIV Treatment Failure and Resistance

  title={A Combined Screening Platform for HIV Treatment Failure and Resistance},
  author={Myres W. Tilghman and Susanne J. May and Josu{\'e} P{\'e}rez-Santiago and Caroline C. Ignacio and Susan J. Little and Douglas D. Richman and Davey M. Smith},
  booktitle={PloS one},
BACKGROUND To develop a low cost method to screen for virologic failure of antiretroviral therapy (ART) and HIV-1 drug resistance, we performed a retrospective evaluation of a screening assay using serial dilutions of HIV-1 RNA-spiked blood plasma and samples from patients receiving >6 months of first-line ART. METHODS Serial dilution testing was used to assess sensitivity of a simple PCR-based assay (targeted at ≥1,000 HIV RNA copies/mL). We created blood plasma minipools of five samples… CONTINUE READING
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