A Chemical Tool for In Vitro and In Vivo Precipitation of Lysine Methyltransferase G9a

@article{Konze2014ACT,
  title={A Chemical Tool for In Vitro and In Vivo Precipitation of Lysine Methyltransferase G9a},
  author={Kyle D. Konze and Samantha G. Pattenden and Feng Liu and Dalia Barsyte-Lovejoy and Fengling Li and Jeremy M. Simon and Ian J. Davis and Masoud Vedadi and Jian Jin},
  journal={ChemMedChem},
  year={2014},
  volume={9}
}
Here we report the design, synthesis, and biochemical characterization of a new chemical tool, UNC0965. UNC0965 is a biotinylated version of our previously reported G9a chemical probe, UNC0638. Importantly, UNC0965 maintains high in vitro potency and is cell penetrant. The biotinylated tag of UNC0965 enables “chemiprecipitation” of G9a from whole cell lysates. Further, the cell penetrance of UNC0965 allowed us to explore the localization of G9a on chromatin both in vitro and in vivo through… 
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