A Cautionary Note on the Use of Split-YFP/BiFC in Plant Protein-Protein Interaction Studies

@inproceedings{Horstman2014ACN,
  title={A Cautionary Note on the Use of Split-YFP/BiFC in Plant Protein-Protein Interaction Studies},
  author={Anneke Horstman and Isabella Antonia Nougalli Tonaco and Kim Boutilier and Richard G. H. Immink},
  booktitle={International journal of molecular sciences},
  year={2014}
}
Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions. BiFC is based on the restoration of fluorescence after the two non-fluorescent halves of a fluorescent protein are brought together by a protein-protein interaction event. The major drawback of BiFC is that the fluorescent protein halves are… CONTINUE READING
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US National Library of Medicine National Institutes of Health

  • PubMed-NCBI
  • Available online: http://www.ncbi.nlm.nih.gov…
  • 2014
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