A CRISPR CASe for high-throughput silencing

  title={A CRISPR CASe for high-throughput silencing},
  author={Jacob Heintze and Christin Luft and Robin Ketteler},
  booktitle={Front. Genet.},
Manipulation of gene expression on a genome-wide level is one of the most important systematic tools in the post-genome era. Such manipulations have largely been enabled by expression cloning approaches using sequence-verified cDNA libraries, large-scale RNA interference libraries (shRNA or siRNA) and zinc finger nuclease technologies. More recently, the CRISPR (clustered regularly interspaced short palindromic repeats) and CRISPR-associated (Cas)9-mediated gene editing technology has been… CONTINUE READING
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A CRISPR CASe for high-throughput silencing

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