A COS-cell-based system for rapid production and quantification of scFv::IgC kappa antibody fragments.

@article{Ridder1995ACS,
  title={A COS-cell-based system for rapid production and quantification of scFv::IgC kappa antibody fragments.},
  author={Ruediger Ridder and Sabine Geisse and Burkhard Kleuser and Petra Kawalleck and Hermann Gram},
  journal={Gene},
  year={1995},
  volume={166 2},
  pages={273-6}
}
Single-chain Fv (scFv) antibody (Ab) fragments were transiently produced in COS-1 cells utilizing a mammalian expression vector featuring a murine immunoglobulin (Ig) light-chain leader sequence for efficient secretion and a murine Ig kappa constant domain (IgC kappa) for detection. Several hundred milliliters of supernatants from large-scale COS cell transfections were sufficient to purify the scFv::IgC kappa fusion proteins by one-step affinity chromatography utilizing an immobilized rat anti… CONTINUE READING

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