A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification

@article{2008ABC,
  title={A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification},
  author={},
  journal={Principles and Technical Aspects of PCR Amplification},
  year={2008},
  pages={9 - 15}
}
  • Published 2008
  • Biology
  • Principles and Technical Aspects of PCR Amplification
In principle, PCR generates large quantities of DNA from a minute amount of nucleic acid starting material using a methodology similar to (but much simpler than) that seen in living cells. For living cells, in vivo DNA synthesis is dependent upon a well defined but complex set of enzymes and co-factors, which have evolved to act in a concerted fashion during the synthetic phase (S-phase) of the cell cycle. In comparison, PCR facilitates in vitro DNA synthesis in a much simpler fashion, making… 
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