A Bowman-Birk-type trypsin-chymotrypsin inhibitor from broad beans.

  title={A Bowman-Birk-type trypsin-chymotrypsin inhibitor from broad beans.},
  author={Xiujuan Ye and T. B. Ng and Ping Fan Rao},
  journal={Biochemical and biophysical research communications},
  volume={289 1},
An isolation procedure comprising affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Toyopearl, and fast protein liquid chromatography on Mono S was used to purify a peptide from broad beans which manifested antifungal activity toward Mycosphaerella arachidicola, Fusarium oxysporum, and Botrytis cinerea. The peptide demonstrated a molecular mass of 7.5 kDa. N-terminal sequence analysis disclosed the identity of the antifungal peptide to be a trypsin-chymotrypsin… CONTINUE READING
31 Citations
0 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 31 extracted citations

Similar Papers

Loading similar papers…