A Bacillus subtilis fusion protein system to produce soybean Bowman-Birk protease inhibitor.

@article{Vogtentanz2007ABS,
  title={A Bacillus subtilis fusion protein system to produce soybean Bowman-Birk protease inhibitor.},
  author={Gudrun Vogtentanz and Katherine D Collier and Michael Bodo and Judy H Chang and Anthony G Day and David A. Estell and Brandy C Falcon and Grant Ganshaw and Alisha Stephens Jarnagin and James T. Kellis and Marc A B Kolkman and Cindy Sin-Yee Lai and Renato Meneses and Jeffrey V Miller and Hans de Nobel and Scott Power and Walter Weyler and David C. Wong and Brian F Schmidt},
  journal={Protein expression and purification},
  year={2007},
  volume={55 1},
  pages={40-52}
}
A fusion protein based expression system was developed in the Gram-positive bacterium Bacillus subtilis to produce the soybean Bowman-Birk protease inhibitor (sBBI). The N-terminus of the mature sBBI was fused to the C-terminus of the 1st cellulose binding domain linker (CBD linker) of the BCE103 cellulase (from an alkalophilic Bacillus sp.). The strong… CONTINUE READING