A B23-interacting sequence as a tool to visualize protein interactions in a cellular context.

@article{Lechertier2007ABS,
  title={A B23-interacting sequence as a tool to visualize protein interactions in a cellular context.},
  author={Tanguy Lechertier and Valentina Sirri and Dani{\'e}le Hernandez-Verdun and Pascal Roussel},
  journal={Journal of cell science},
  year={2007},
  volume={120 Pt 2},
  pages={265-75}
}
We report the characterization of a nucleolar localization sequence (NoLS) that targets the green fluorescent protein (GFP) into the granular component (GC) of nucleoli. This NoLS interacts in vitro specifically and directly with the major nucleolar protein B23 and more precisely with the region of B23 including the two acidic stretches. The affinity of NoLS for B23 is stronger than that of the HIV-1 Rev protein in vitro. Moreover, B23-NoLS interaction also occurs in vivo. Indeed, (1) NoLS… CONTINUE READING
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