A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

@article{Krkkinen2008A9F,
  title={A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells},
  author={Hanna-Riikka K{\"a}rkk{\"a}inen and Hanna Pirita Lesch and Antti I M{\"a}{\"a}tt{\"a} and Pyry I. Toivanen and Anssi Juhani M{\"a}h{\"o}nen and Miia M. Roschier and Kari Juhani Airenne and Olli H. Laitinen and Seppo Yl{\"a}-Herttuala},
  journal={BMC Research Notes},
  year={2008},
  volume={2},
  pages={63 - 63}
}
BackgroundBaculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications.FindingsWe describe here protocols which adapt baculovirus generation into 96-well format.ConclusionThe established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are… CONTINUE READING
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