A 96-well DNase I footprinting screen for drug–DNA interactions

  title={A 96-well DNase I footprinting screen for drug–DNA interactions},
  author={Tom Ellis and Davidr . Evans and Christopher R. H. Martin and John A. Hartley},
  journal={Nucleic Acids Research},
  pages={e89 - e89}
The established protocol for DNase I footprinting has been modified to allow multiple parallel reactions to be rapidly performed in 96-well microtitre plates. By scrutinizing every aspect of the traditional method and making appropriate modifications it has been possible to considerably reduce the time, risk of sample loss and complexity of footprinting, whilst dramatically increasing the yield of data (30-fold). A semi-automated analysis system has also been developed to present footprinting… 

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